Chromatography and Analytical Techniques

Molecular Exclusion Chromatography

Molecular exclusion chromatography also called or named as Size exclusion chromatography (SEC) separates molecules depending on their molecular size by filtration through a gel. The gel consists of spherical beads containing pores of a particular size distribution. Separation techniques occurs when molecules present in the mixture, of different sizes are included or removed from the pores within the matrix. Small molecules and large molecules are separated based on entering in to the pores. Consequently, molecules separate based on their size as they pass through the column and are eluted in order of decreasing molecular weight (MW)