Chromatography and Analytical Techniques

Capillary Electrophoresis

Capillary electrophoresis (CE) is a high-efficiency separation technique widely used for analyzing small ions, biomolecules, and chiral compounds. Its core principle is the differential migration of charged analytes through a capillary under an electric field, offering excellent resolution and rapid analysis with minimal sample and reagent consumption. Recent advancements in CE include microchip-based formats, improved capillary coatings to minimize adsorption, and coupling with mass spectrometry (CE-MS) for enhanced detection capabilities. These innovations have broadened its applications in pharmaceutical analysis, forensic science, clinical diagnostics, and proteomics. CE is especially beneficial for analyzing proteins, peptides, and nucleic acids, making it a key tool in biotechnology and bioanalytical labs. The technique’s compatibility with aqueous buffers and low environmental impact align well with green chemistry principles. Automated systems and multiplexing have further increased throughput and reproducibility, making CE a practical solution for high-volume laboratories. This topic highlights how capillary electrophoresis stands as a cost-effective, robust, and environmentally friendly technique with growing relevance across diverse scientific and industrial sectors.