Chromatography and Analytical Techniques

Affinity Chromatography

Affinity chromatography is one of the important separation techniques based on a specific binding interaction between an immobilized ligand and its binding partner. Affinity chromatography is a type of liquid chromatography that makes use of interactions for the identification and specific analysis of sample components. Examples include antibody and antigen, enzyme and substrate, and enzyme and inhibitor interactions. The degree of purification can be quite high in a purification strategy. This present track provides an knowledge of affinity chromatography of proteins. Immune affinity chromatography is one of the important types of affinity chromatography. Immune affinity chromatography technique is more commonly performed for the identification, quantification, or purification of antigens