Chromatography

Advancement in Chromatography

Chromatography, different method for identifying and separating the components, or solutes, of a mixture on the basis of the relative amounts of each solute distributed between a moving fluid streams, called the mobile phase, and a contiguous stationary phase. Liquid-chromatography is one of the mostly employed and useful techniques in pharmaceutical industry for sample preparation. Another one is electrophoretic separation technique designed for rapid and selective sample analysis. Immunoaffinity chromatography (IAC) is a different method of Liquid Chromatography in which the stationary phase consists of an antibody or it might include antibody-related reagent. This technique involves a unique sub type of affinity chromatography, in which a biologically related binding agent is taken for the selective separation or analysis of a target compound. Capillary electrophoresis is other method which identifies and distributes ions depending on their electrophoretic mobility with the use of an applied voltage. Compact mass spectrometer is widely used in the food safety, water purity and clinical diagnosis.

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