Chromatography and Analytical Techniques

Adsorption Chromatography

Adsorption chromatography is definitely one of the earliest types of analytical method of separation technology. Adsorption means a physical connection between the compound and the particles of stationary phase. It utilizes a mobile liquid or gaseous phase that is adsorbed onto the top most layer of an immobilized solid phase. Column chromatography has been employed to separate vitamins, steroids, hormones, and alkaloids and to determine the amounts of these substances in samples of body fluids .The different separation techniques involved are column chromatography, thin layer chromatography and liquid chromatography.