Chromatography and Analytical Techniques

Protein and Peptide Analysis

The characterization and quantification of proteins and peptides are crucial in biopharmaceutical development, proteomics, and biomedical research. Chromatographic techniques such as reversed-phase liquid chromatography (RPLC), ion-exchange chromatography (IEX), size-exclusion chromatography (SEC), and hydrophilic interaction chromatography (HILIC) are extensively used for separating complex protein mixtures, analyzing post-translational modifications, and determining protein purity. Coupling with high-resolution mass spectrometry (LC-MS) further enables in-depth identification and structural elucidation of peptide fragments. Advancements in stationary phases, bioinert columns, and online sample preparation methods have significantly enhanced analytical efficiency and reproducibility. Automation, multi-dimensional chromatography, and micro/nanoflow LC systems have become standard in high-throughput labs. These developments are critical in quality control of biotherapeutics, including monoclonal antibodies and vaccines. Moreover, robust methods are necessary for compliance with regulatory standards governing biologics. This session explores state-of-the-art techniques and applications of chromatography in protein and peptide analysis, focusing on innovations that ensure reproducibility, sensitivity, and comprehensive coverage in complex biological systems.