Chromatography and Analytical Techniques

Ion Exchange Chromatography

Ion exchange chromatography (IEX) separates molecules present in the mixture based on differences in their net surface charge. Ion exchange chromatography playing an important role in the separation and purification of biomolecules. Today, IEX is one of the most frequently used separation techniques for purification of amino acids, proteins, peptides, and nucleic acids. Ion Exchange Chromatography depends up on charge-charge interactions between the proteins in the sample and the charges immobilized on the resin of choice. Loading samples in buffers of low ionic strength makes ion exchange chromatography an excellent purification step after hydrophobic interaction chromatography