Chromatography

Biography

Biography: Berkant Kayan

Abstract

Drug active ingredients are containing one or more chemical substances that cause physiological changes in vivo and substances that are responsible for the beneficial health effects experienced by patients. Drugs are usually grouped according to their physiological effects and antidepressants one of the most frequently used of this group. In this study fluoxetine and sertraline as selected model molecules from antidepressant active agent, which have environmental as well as pharmacological and biological importance. For this purposes naphthylamine attached poly (HEMA-MAH)(PHEMAH) (poly(hydroxyethyl methacrylate-N-methacryloyl-(L-histidine)-methylester) microspheres, which have  different particle sizes will be synthesized and used as the column filling material (stationary phase) in high temperature liquid chromatography which is the one of the green chromatography techniques and determination of the method development and optimization conditions for the synthesized column filler will be conducted by using chemometrics application of response surface methodology. Many analytical methods such as High Performance Liquid Chromatography, Liquid Chromatography-Mass Spectrometry/Mass Spectrometry, Gas Chromatography-Mass Spectrometry are used in literature for the chromatographic separation of the active ingredients of antidepressants. Among the used these techniques, the high performance liquid chromatography (HPLC) shows the most effective separation technique. While RPLC (reversed phase liquid chromatography) is a popular analytical technique used today, organic solvents are required in traditional RPLC and an enormous amount of organic solvent is consumed worldwide for just chromatographic separation. The organic solvents is used in HPLC are very hazardous and dangerous for environmental and human health, as well as expensive in term of both purchasing and waste disposal costs. Industrial wastewater pollution is being one of the major and perhaps most importantly problem resulting from uncontrolled of technology in developing countries. Therefore, separation, purification and determination methods are being more important and “nature-friendly” products and dissemination of related researchers is great importance for use as solid/solid phase of determination methods due to environmental concerns. High temperature liquid water chromatography technique is used the minimum level of organic solvent in which worldwide attention recently. At the same time, in order to be successful of separation in HTLC, synthesized a new generation of stable and resistant filler column and is also an important application be so successful at elevated temperature conditions. The systematic studies are expected rapid, accurate, is expected to reach accurate and reliable results as the requirement of a scientific research.