World Congress on Chromatography September 21-23, 2016 Amsterdam, Netherlands

BuÄŸra DAYI has completed his license degree. He studies at Pamukkale University, Department of Chemistry, Biochemistry subdivision as a master student. He works Environmental biotechnology, Waste water bioremediation and Dye removal.

Wastewater from the textile industry is one of the most problematic to treat due to its color, high chemical oxygen demand (COD), biochemical oxygen demand (BOD), suspended solids, turbidity and toxic compounds. The chemical composition of the textile effluents has changed rapidly due to a shift in the consumer preferences, the most significant of these being the popularity of cotton fabrics and bright colors leading to greater usage of synthetic reactive dyes and azo dyes[1,2]. By far the single class of micro-organisms most efficient in breaking down synthetic dyes is the white-rot fungi. These fungi constitute a diverse eco-physiological group comprising mostly basidiomycetous and to a lesser extent litter-decomposing fungi capable of extensive aerobic lignin depolymerisation and mineralisation. The mechanism of fungal decolorization mainly involves two aspects, biodegradation and biosorption. The biodegradation capability of fungi is due to their extracellular, non-specific and non-selective enzyme system[3].rnIn our experience, white rot fungus M. esculenta was immobilized on to three different support materials (polyurethane, kaolin, cellulose). Biodecolorization of mixed dyes were investigated and the data were compared for all immobilized cells. Polyurethane was selected as immobilization support material for the best dye removal (dye concentration: 10 mgL-1, 97,78%) in agitated system. At the end of the biodecolorization, samples (10 mgL-1) was analyzed to FT-IR and UV spectrum for identify any possible metabolites. When the obtained datas were examined, any metabolites was not found. As a result, immobilized M.esculanta (on to Polyurethane) could be used the wastewater bioremediation.

Hatice A Akdogan has completed her PhD and works at the Pamukkale Umiversity in the Department of Chemistry, Biochemistry subdivision as an Associate Professor. She studies Environmental Biotechnology, Water and Soil Bioremediation, Chromatographic Monitoring of some organic contaminants during microbial biodegradations, microbial enzymes and their roles.

The utilization of immobilized cells has shown potential in several bioprocesses including wastewater treatment. Immobilization can be considered as the natural state for several microorganisms; for example, most fungi tend to attach firmly to natural surfaces. Therefore, it is not surprising that artificially immobilized microorganisms can produce extracellular secondary metabolites. In industrial operations, immobilized microbial cell systems could provide additional advantages over freely suspended cells such as simple reuse of the biomass, easier liquid-solid separation and minimal clogging in continuous-flow systems. The immobilization of microorganisms can be defined as any technique that limits the free migration of cells. Basically, there are two types of cell immobilization: entrapment and attachment. In the former, the organisms trapped within the interstices of fibrous or porous materials are physically restrained by a solid and porous matrix. Our research focused on the immobilization of Coprinus plicatilis on kaolin, Ca-alginate and gelatin. 3 or 4 age cells and different amount of cells were used for immobilization studies. To the best of our knowledge, the results showed that gelatin was chosen as a support material because it is a natural material with a higher immobilization capacity and is less expensive.

Kahtan J Hasoon has obtained his BSc in Pharmaceutical Sciences from College of Pharmacy, Baghdad University. He obtained his MSc in Pharmaceutical Analysis from Herriot-Watt University, UK. He has been a Member of Academic Staff of Al-Mustansiriya University. Now he is a Lecturer in the Al-Rasheed University College, and as a Technical Consultant in SAFA Pharmaceutical Industries Co., Al-Safa Group.

Telmisartan is an angiotensin ІІ type and is used as antihypertensive drug. It poses poor solubility which leads to low bioavailability in blood stream so that; this problem guides many scientists to work in improvement of telmisartan dissolution of its solid dosage forms. The present work shows the formulations of telmisartan as tablet with high enhancement degree in their dissolutions and stability in addition to the improvement in the physical characters of the tablet dosage forms. The formulations of telmisartan were prepared in consideration of manufacturing conditions rather than laboratory productions as it is prescribed over many previous academic papers. The manufacturing procedure of the tablet according to this new formulation is simple and readily applicable in pharmaceutical industries. The method depend on turning the telmisartan to amorphous crystals by mixing with solubilizing polymer and mixed with prepared DC excipients to be compressed as tablets. The DC excipients were modified during the preparation to act as alkalizing agent which in turn enhanced the dissolution rate of the tablets. The physical properties of the powder of formulations were evaluated and it gave an excellent degree of flowability and compressibility while, the compressed tablets showed fast disintegration time and very low friability percent. In addition the dissolution profiles of the produced tablet were more than 85%. For a comprehensive evaluation of this formulation procedure of tablets, a stability indicating method of analysis was developed by using reversed phase HPLC technique to follow the expected changing that might occur on storage of the product (tablets) at accelerated conditions of storage. The HPLC method was able to detect the degradation products of telmisartan in deliberately degraded sample and the produced telmisartan tablets which were stored at accelerated conditions showed good stability.

Nico Anders has been working in the field of analysis and renewables since 2009. He has obtained his PhD at the TU Braunschweig in the group of Prof. Dr. Vorlop in technical chemistry. Since 2013, he is working as junior research group leader in the Aachener Verfahrenstechnik at the RWTH Aachen University. The research interests of Nico Anders are analysis of lignocellulosic biomass, green analytical chemistry, conversion of lignocellulosic biomass and chromatographic separation.

Hydrolyzates from lignocellulosic biomass are a complex mixture of soluble monomeric and oligomeric fragments. These fragments are degradation products from all four natural polymers cellulose, hemicellulose, pectin and lignin. As the polarity and size varies for all fragments depending on the origin, the analysis requires more than one analytical method and a broad know how. Anion exchange chromatography (AEC) has a great potential as the measurement is based on the ability of producing anions from soluble lignocellulosic biomass derived fragments. Thus, the aim of this study was to characterize hydrolyzates from different biomasses for soluble biomass derived degradation products with AEC. Therefore, the parameters column temperature (30°C to 50°C), eluent composition and chromatographic run time were investigated for separation of the biomass derived degradation products and short chromatographic run time. The final method was set to a column temperature of 40°C, an eluent flow of 1mL/min and an eluent consisting of sodium acetate and sodium hydroxide as well as ultrapure water. Using the newly developed method a run time of 70 min could be realized for degradation products from all natural polymers. Additionally, the limit of detection in the range of 0.014 mg/L for 2, 6-dimethoxyphenol and 21.9 mg/L for 4-methoxybenzyl alcohol allows for a simultaneous determination of lignin derived compounds beside the high glucose concentrations. In consequence AEC was used to characterize hydrolyzates from 17 lignocellulosic biomasses for soluble compounds derived from cellulose, hemicellulose, pectin and lignin.

Petrovich Olga Mikhailovna is a Chemical Engineer at R & D Centre, Polyplast Novomoskovsk Ltd, Russia.

The technical polymethylene naphthalene sulfonates (TPNS) are used in different industries. TPNS are thought to be mixture of polymethylene naphthalene sulfonates with wide molecular weight distribution (MWD) parameters and different span of the average molecular weights. MWD and average molecular weights determine the field of application of this product. For evaluation of TPNS MWD characteristics, two chromatographic methods are used gel permeation (GP) and thin layer (TL) chromatography as well as extraction-precipitation (EP) procedure. All these methods have drawbacks. For EP, the drawbacks are the duration of analysis; low reproducibility and low selectivity of fractionating and; for GP and TL chromatography, the drawbacks are the poor informativity because of the insufficient separation and complexity of the quantitative assessment of obtained data. We believe that reversed phase high performance liquid chromatography (RP-HPLC) with spectrophotometric detector is an interesting and advanced method. The systematic researches of TPNS MWD by means of this method are very rare. The objective of this research is to develop the methodology of HPLC and to compare it with known methods of the assessment of TPNS MWD. The capabilities and advantages of the methodology of HPLC are demonstrated on the TPNS samples for different industries. The high reproducibility and informativity of the method are revealed compared to exist methods. New method allows to separate, identify and quantify the full fraction makeup of TPNS. The characteristics of MWD are estimated reliably.