Taghreed Alsufyani
Taif University, KSA
Biography
Field experiments usually give ecologically relevant results contributing to understand the ecosystem deeply. In marine research, aquacultures are one of the most popular procedures used to carry out fi eld experiments (fi gure a). In this study, the aquaculture of the green macroalga Ulva mutabilis was inoculated for the fi rst time with freshly induced gametes (7-day old germlings) to culture large volume (200 L) with small axenic gametes. Two sets of aquaculture: defi ned community (inoculated with axenic gametes of U. mutabilis and two associated bacteria: Roseovarius sp. strain MS2. and Maribacter sp. strain MS6), and undefi ned community (inoculated of U. mutabilis axenic gametes only). In defi ned community U. mutabilis showed a healthy growth and development (fi gure b) whereas in undefi ned community U. mutabilis lost its ability for growing and developing and formed only callus-like colonies (fi gure c). Multivariate statistics of the GC/MS and LC/MS analyses along with acquisition of biological metadata revealed that the waterborne metabolites in defi ned community were aff ected qualitatively and quantitatively by the growth phases of U. mutabilis as was proven in previous study of bioreactor cultures.