Seyed Jamaleddin Shahtaheri
Tehran University of Medical Sciences, Iran
Title: Optimization of liquid-liquid micro-extraction technique for trace determination of pesticide diazinon before HPLC analysis
Biography
Biography: Seyed Jamaleddin Shahtaheri
Abstract
A state-of-the-art isotope dilution LC-MS/MS method was developed for the simultaneous measurement of nine steroids. Diurnal reference intervals were determined and published. The method was then employed to assess patients with different forms of adrenal disease. We found that after ACTH stimulation the two best markers of adrenal reserve were 11-DOC and corticosterone. Studies are currently underway further validating this statement. Cortisol should no longer be regarded as the most reliable test to perform after ACTH stimulation of the adrenals. Our studies with the new markers reveal a possible prior misclassification rate of 20%.