Károly Héberger
Hungarian Academy of Sciences, Hungary
Title: Separation selectivity of liquid chromatographic columns. A comparison by nonparametric methods
Biography
Biography: Károly Héberger
Abstract
There are two legitimate aims for column selection: i) to determine similar ones to an existing one, and ii) to find diverse (orthogonal) one(s) for optimal separation. Several different methods have already been elaborated to compare selectivity of chromatographic columns. All comparisons realize empirical approaches and based on measuring retention data of several well-chosen test compounds. Proper multivariate analyses can find similarities and differences in retention behavior of test compounds and stationary phases. As an illustration we adopted Wilson et al.’s data of 67 test compounds and ten highly similar columns (C18-bonded silica stationary phases).1 The inherent characteristic groupings by physical properties were revealed with correct statistical tests and several independent methodologies.
Generalized pair correlation method (GPCM)2 and sum of (absolute) ranking differences (SRD)3,4 unambiguously show the same ranking pattern. The clustering by SRD is delivered to the reference. Therefore, all columns have been chosen as gold standard once and only once. (Comparison with One VAriable at a Time.5 All lines of boxes correspond to an SRD ordering always with a different reference column (Figure 1). COVAT heatmaps show destroying the true pattern if the HSM evaluation is used.
The ranking (clustering) pattern of chromatographic columns based on retention data (logk values) of 67 compounds, and selectivity parameters of hydrophobic-subtraction model (HSM) provided various column groupings. Loss of information is inevitable if using the HSM data handling. Processing of retention data resulted in patterns that are consistent with differences in the columns’ physicochemical parameters, whereas HSM results are deviating to a higher or lesser degree, depending on the particular chemometric approach.
GPCM, SRD and COVAT procedures can be carried out on any data sets partially and on the whole to select the most similar and dissimilar columns, though our calculations were completed to the data set of Wilson et al.1