Chromatography

Biography

Biography: Mellissa Graewert

Abstract

Small angle X-ray scattering (SAXS) is a universal and powerful method to analyze proteins and other macromolecules in
solution, in a broad range of sizes and conditions. Th e synergistic improvement in hardware as well as soft ware over the
last decade has transformed SAXS into a high-through put technique, which became highly attractive for the pharmaceutical
industry. SAXS provides direct insights in the quaternary state; however, it is oft en hampered by inherent sample polydispersity.
At EMBL’s P12 beamline (@Petra III, DESY, Hamburg, Germany), we are developing novel in-line purifi cation systems such as
the implementation of an extended size exclusion chromatography set-up for the parallel biophysical and SAXS characterization
on separated components. Th e eluting protein is suffi ciently concentrated and pure, so that SAXS data can be directly collected
and used for structural and biophysical studies. Modes of access to this set-up (including European funded translational
activities such as iNEXT and industrial service provision) are discussed.