Chromatography

Biography

Biography: Tony Q Yan

Abstract

This presentation discusses immobilized polysaccharide stationary phases with extended range of solvents for enantiomer resolution of select amino acids and derivatives using supercritical fluid chromatography (SFC) and high performance liquid chromatography (HPLC).  Baseline resolution is achieved for selected amino acids and derivatives using this approach. The combination of an extended solvent (normally an aprotic solvent) in the presence of methanol and a suitable additive such as water, acid and base are critical for retention and resolution of amino acid pairs on immobilized columns. The amino acid separations are also achieved on the coated polysaccharide phases with alcohol based solvents in the presence of acid and base.  In addition, the use of cyclofructan and polysaccharide phases for amino acids and small peptides separations along with the use of reversed phase chiral stationary phase for very polar compounds, acids and diacids, are also discussed.