High Performance Liquid Chromatography (HPLC) is different and another type of column chromatography that pumps a sample mixture or analyte in a solvent at high pressure through a column with chromatographic packing material. HPLC has the ability to analyse, and separate compounds that would be present in any sample that can be dissolved in a liquid in trace concentrations. Because of this advantage, HPLC is used in a variety of industrial and scientific applications, such as pharmaceutical industry, environmental, forensic science, and chemicals. High Performance Liquid Chromatography has brought lot of advantages in the department of food analysis and also in the analysis of various fat soluble vitamins. HPLC is also used in DNA fingerprinting and bioinformatics.

Gas chromatography is a generally a word used to give information about the group of analytical separation techniques used to analyze volatile materials in the gas phase. In this chromatography, the components of a sample are dissolved in a solvent and vaporized which results in the separation of analytes by differentiating the sample between two phases. Gas chromatography is one of the important types of chromatography that does not use the mobile phase for reacting with the analyte. If the stationary phase consists of solid adsorbant, termed gas-solid chromatography, or a liquid on an inert support, termed gas-liquid chromatography (GLC). Gas Chromatography Mass Spectrometry (GC-MS) is the advanced technique of Gas Chromatography. Gas chromatography can be used in many different fields such as pharmaceutical industry, cosmetology and even environmental toxins. In Pharmaceutical Industry, GC is widely used because of its high efficiencies of separation power and also due to the precision and accuracy of the data from quantitative analyses of very complex mixtures.

Chromatography utilizes phase equilibrium partitioning principles to isolate proteins, nucleic acids, or little particles in complex blends in view of their varying connections with a stationary phase and a mobile phase. There are two main types of chromatography: Liquid chromatography (LC) and gas chromatography (GC). Both LC and GC can be utilized for either preparative or investigative applications. Chromatography strategies can likewise be ordered by the chromatography media chemistry: the sorts of physicochemical associations used to segment components into the stationary and mobile phases thus accomplish separation.

Analytical chromatography is routinely utilized as a part of industry and the scholarly world for partition, quantitation and ID of chemical or organic mixes. Chromatographic examination is additionally essential in the generation of pharmaceuticals, chemicals or food and beverages. Analytical chromatography is utilized to decide the existence and possibly also the concentration of analyte(s) in a specimen. A reinforced stage is a stationary stage that is covalently attached to the support particles or to within mass of the section tubing. A chromatogram is the visual yield of the chromatograph.

A Hyphenated technique is mix or coupling of two distinctive analytical techniques with the assistance of proper interface. Mostly chromatographic techniques are consolidated with spectroscopic techniques. The term hyphenated methods ranges from the blend of separation-separation, separation-identification & identification-identification techniques. The hyphenation of these strategies prompts better examination of the segments. Hyphenated methods indicate specificity and sensitivity.

The method of separation is united type of unit procedure in most of the modern pharmaceutical, chemical and other process plants. Newer separation processes, like super critical fluid chromatography, paper chromatography hybridization assay chromatographic separation, etc., are gaining importance in modern days plants as novel separation processes. The Hyphenated Technique is increased and made advanced by uniting of a separation technique and an on-line spectroscopic detection technology. In this session latest update in the advancement of different hyphenated techniques, e.g., LC-FTIR, LC-NMR, CE-MS, GC-MS, Liquid Chromatography MS, etc. in the method of pre-isolation identification of crude extracts or fractions from different natural sources, separation and  identification of natural products, chemotaxonomic studies and DNA fingerprinting are included.

Both comprehensive two-dimensional gas chromatography (GCxGC) and comprehensive two-dimensional liquid chromatography (LCxLC) have developed as capable tools for expanding the peak limit of a solitary chromatographic analysis. Using orthogonal chromatographic systems, analytes can be isolated by two unique, successive maintenance systems; this procedure takes into consideration analytes to be isolated from different compounds that would regularly co-elute in a solitary dimensional separation. Endeavors at NIST are being coordinated toward the investigation of issues identified with quantitation by both GCxGC and LCxLC, esteem task of complex matrix Standard Reference Materials (SRMs), and non-targeted analysis.

Special techniques in analytical chemistry also play an important role in the analysis of many chemical compounds. Physical separation techniques are the most common, least expensive and safest. Component separation is also relatively easy but tends to be more expensive. The specialty chemical and pharmaceutical industries are the primary users of component separation techniques. Component separation is only now being applied to hazardous wastes and many processes are still in the demonstration stage. Chemical separation techniques are the most expensive and pose the greatest potential hazard. In Hydrophobic Interaction Chromatography (HIC) the proteins are separated according to differences in the amount of exposed hydrophobic amino acids.

The separation mechanism relies on contrasts in polarity between the diverse encourage segments. The more polar a particle, the all the more firmly it will be adsorbed by a polar stationary stage. Additionally, the more non-polar a particle, the all the more strongly it will be adsorbed by non-polar stationary phase. Amid a surface adsorption chromatography process, there is competition for stationary phase adsorption sites, between the materials to be separated and the mobile phase. Encourage atoms of low extremity invest relatively more time in the mobile phase than those particles that are exceptionally polar, which are held longer. In this way the segments of a blend are eluted arranged by increasing polarity.

Electro-migration techniques give the separation of analysed sample components attributable to outer voltage creating electro-kinetic phenomena electrophoresis and electro-osmosis. Considering the moderately large number of parameters managed amid electrophoretic investigations, it is basic to know their impact on the accomplished separation of analytes. Electro migration techniques, because of their tremendous explanatory potential, are broadly connected in the assurance of different substances. These techniques give partition of segments of an analysed sample owing to external producing electro-kinetic phenomena electrophoresis and electro-osmosis.

The Chromatography industry is showing growth rapidly, with value expected to hit 228 billion dollars by 2016, up from 164 US billion dollars in the year of earlier 2010, growing annual growth of nearly 7, according to a recent industrial marketing research report. Geographically, global separation technique market has been segmented into four areas namely, North America, some parts of European region, Asia-Pacific and Rest of the World. The global gas chromatography market, valued at $2,583.6 million in earlier 2014, is possibility to march up to $3,605.1 US million by 2019 at a CAGR of 6.9%.This global market report categorizes the market into 3 wide segments, like, Instrumentation, Accessories & Consumables, and Reagents.

Biomedical Chromatography is a process in which a chemical mixture carried by a liquid or gas is separated into components as a result of differential distribution of the solutes as they flow around or over a stationary liquid or solid phase. There are two main categories of chromatography: preparative and analytical. A sample to be separated, when placed on the stationary section, will gradually move along in the same direction as the mobile phase. If a sample compound (or analyte) has no interaction with the stationary phase, it will run right through and come out of the system (elute) at the same rate as the mobile section. On the opposite hand, if an analyte has no interaction with the mobile phase, it will stick on to the stationary phase and never elute. Neither of these are good outcomes.

Electrophoresis is used to characterize proteins, nucleic acid and any sub cellular particles. Main principle is based on the charge of the particles. Charged particles migrate in the applied electric field. Positively charged ions migrate towards a negative conductor and negatively-charged ions migrate toward a positive conductor.